First pregnancy achieved from human vitrified oocytes
DOI:
https://doi.org/10.18597/rcog.595Keywords:
oocyte, vitrification, survival, viability, pregnancy, ICSI, cryopreservation, cryobankAbstract
Among its multiple applications, vitrification of human oocytes has demonstrated to be highly effective in the handling of a patient with OHSS, who was under treatment for secondary infertility.
Objective: to demonstrate the efficiency of oocyte vitrification applied to metaphase II oocytes (MII), of women presenting OHSS.
Procedure: MII oocytes from a patient with OHSS were vitrified, using the cryotop technique with ethylenglycol, dimethylsulfoxide, and sucrose as cryoprotectants agents. Two months after, once the clinical stage of the patient was normalized, the oocytes were thawed and underwent ICSI; 24 hours later, fertilization was evaluated and zygotes were maintained under in vitro culture conditions during 72 hours, time of intrauterine embryo transfer.
Results: vitrification technique with cryotop demonstrated to be an effective tool for preservation of oocytes instead of freezing embryos due to all the ethical, moral, legal and religious points of view concerning preservation.
Conclusions: vitrification with cryotop provides high rates of survival, fertilization, embryo development and ongoing normal pregnancies, offering a new alternative for handling OHSS.
Author Biographies
Elkin Lucena
Diana Patricia Bernal
Carolina Lucena
Alejandro Rojas
Sandra Mojica
Ángela María Saa
Zulma Suárez
Abby Morán
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