Genotyping the human papilloma virus (HPV) in Cytobrush samples taken from patients attending different hospitals in Bogotá (Colombia) and evaluating agreement between two PCR-based methods
DOI:
https://doi.org/10.18597/rcog.253Keywords:
papilloma virus, cervical dysplasia, pathology, molecular biologyAbstract
Objective: genotyping HPV in samples taken from patients having different histopathological degrees of cervical injury.
Materials and methods: this was a cross-sectional study of cervical scrape samples taken from females having different degrees of histopathological cervical injury in Bogotá during 2009. Luminex® xMAP® was used, which is a highly sensitive, reproducible and automated technique, as well as reverse line blot (RLB).
Results: infections caused by HPV types 16 and 18 were the most frequently found types of pathology of the cervix. Mixed infections predominated (78.04%); amongst cases having negative pathology, 85.7% was found for cervical intraepithelial neoplasia (CIN) I, 81.25% for CIN II, 78.78% for CIN III and 71.42% for cervical cancer. Agreement between both methods was 67%.
Conclusion: infection by VPH types 16 and 18 predominated in the population being studied.
Author Biographies
Dabeiba A. García
Markus Schmitt
Ángel Cid-Arregui
Marcos Castillo
Ignacio Briceño
Fabio A. Aristizábal
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