Polimerase Chain, polimerase for the detection Toxoplasma Gondii in amniotic fluid, report of 534 Cases
DOI:
https://doi.org/10.18597/rcog.743Keywords:
Toxoplasmosis, intrauterine infection, PCR, Amniotic FluidAbstract
Infection with Toxoplasma gondii during pregnancy may result in fetal damage this occurs in 9 of cases, when the mother acquires the infection during the first trimester. If infection ocurrs during the second trimester the fetal infection is about 27% and increase up to 59% when the infection occurs in the third trimester. Fetal infection produces serious secuelae. The diagnosis of intrauterine infection based on immunologic studies, which are of difficult interpretation. This article describes our experience with Polymerase Chain Reaction in amniotic fluid as predictor of fetal infection in pregnant women with immunologic evidence of active infection. From July 1996 to September 2000 we have studied 534 amniotic fluid samples and found 7.1% positive cases. The gestational age were: 12-15w: 6.5%; 16-19w: 16.1%; 20-23w: 20%; 24-27w: 21.5%; 28-31w: 17.6%; 31-35 w: 12.7%; and 36-39 w: 1.7%. Of the 38 positive cases, 20 are clinically normal; one child has visual problems and other has mild psychomotor retardation. Of the other 16 cases, there are 4 fetal deaths; 11 lost follow-up and one hasn't born yet. PCR is a sensitive a specific method to confirm fetal infection, in order to initiate intrauterine therapy, with better prognosis.Author Biographies
Alejandro Giraldo
Dora Fonseca
Fred Wilson Lozano
Jorge E. Orjuela
Andrés Gutiérrez
Ariel Iván Ruiz
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