Evaluating a Toxoplasma IgG avidity ELISA test for diagnostic purposes during pregnancy and correlating it with Toxoplasma IgM and IgA in the Biomedical Research Centre's laboratory at the Universidad del Quindío, 2008
DOI:
https://doi.org/10.18597/rcog.404Keywords:
toxoplasmosis, avidity test, ROC curve, sensitivity, specificityAbstract
Aims: determining the cut-off value for a homebased ELISA assay for IgG avidity at the Biomedical Research Centre at the Universidad del Quindío and correlating the results from pregnant females'serum samples with IgM and IgA test results.
Material and methods: 17 patients having known infection dates were studied to determine the receptor operator curve (ROC) for a home-made avidity assay. 91 sera samples from pregnant females were also analysed at the same laboratory for IgM and IgA anti Toxoplasma antibodies.
Results: 100% sensitivity and 88% specificity were obtained by using a 30% avidity cut-off value for identifying infections acquired less than 4 months beforehand. 63% of the 91 pregnant females had less than 16 weeks' gestation at the time of examination and three of the patients had low avidity tests (<30%). Only one case had avidity less than <30% after 8 months' infection. The avidity test was requested before the 16th week of the gestational period for most of the patients.
Conclusions: a 30% cut-off identified sera containing infections having less than 4 months' evolution.
Author Biographies
Elizabeth Torres Morales
Jorge Enrique Gómez-Marín
References
Nissapatorn V, Lee C, Kia F, Lleong C, Mahmud R, Abdullah KA. Toxoplasmosis in HIV/AIDS patients: a current situation. Jpn J Infect Dis 2004; 57:160-5.
Wong SY, Remington JS. Toxoplasmosis in pregnancy. Clin Infect Dis 1994;18:853-62.
Montoya JG, Liesenfeld O. Toxoplasmosis. Lancet 2004;363:1965-76.
Remington JS, McLeod R, Thulliez P, Desmonts G. Toxoplasmosis. En: Remington JS, Wilson CB, Baker CJ. Klein JO. (eds.) Infectious diseases of the fetus and newborn infant. 6th ed. Philadelphia: WB Saunders; 2006. p. 947-1092.
Rosso F, Agudelo A, Isaza A, Montoya JG. Toxoplasmosis congénita: aspectos clínicos y epidemiológicos de la infección durante el embarazo. Colomb Med 2007;38:316-37.
Hedman K, Lappalainen M, Seppala I, Mäkelä O. Recent primary toxoplasma infection indicated by a low avidity of specific IgG. J Infect Dis 1989; 159:736-9.
Remington J, Thulliez P, Montoya J. Recent developments for diagnosis of toxoplasmosis. J Clin Microbiol 2004;42:941-5.
Cozon GJ, Ferrandiz J, Nebhi H, Wallon M, Peyron F. Estimation of the avidity of immunoglobuling for routine diagnosis of chronic Toxoplasma gondii infection in pregnant women. J Clin Microbiol Infect Dis 1998;17:32-6.
Camargo ME, Silva SM, Leser PG, Granato CH. Avidez de anticorpos IgG específicos como marcadores de infecçâo primaria recente pelo Toxoplasma gondii. Rev Inst Med Trop Sao Paulo 1991;33:213-8.
Maekelt A, Mauriello L, Diaz MP. Evaluación de la prueba ELISA-Avidez-IgG como inmunodiagnóstico serológico de la infección toxoplasmósica reciente. RFM 2000;23:149-56.
Montoya JG, Huffman HB, Remington JS. Evaluation of the immunoglobulin G avidity test for diagnosis of toxoplasmic lymphadenopathy. J Clin Microbiol 2004;42:4627-31.
Gómez JE, Ruiz B, Silva P, Beltrán S, Cortés J, Montoya J, et al. Guía de práctica clínica para toxoplasmosis durante el embarazo y toxoplasmosis congénita en Colombia. Infectio 2007;11:1-13.
Montoya MT, Gómez Marín JE, Loango N, Castaño JC, Marx C, Foudrinier F, et al. Utilidad de dos pruebas serológicas para IgA humana antitoxoplasma como pruebas de referencia para toxoplasmosis materna reciente. Acta Med Colomb 1998;23:275-82.
How to Cite
Downloads
Downloads
Published
Issue
Section
| Article metrics | |
|---|---|
| Abstract views | |
| Galley vies | |
| PDF Views | |
| HTML views | |
| Other views | |
