Comparing early cleavage and pronuclear state as reference parameters for inducing pregnancy in embryo implantation patients in Cali, Colombia
DOI:
https://doi.org/10.18597/rcog.328Keywords:
fertilisation, cleavage, embryo implantation, syngamy, pronuclear, inseminationAbstract
Introduction: some authors have proposed that non-invasive embryo implantation methods (based on early cleavage or pronuclear stage for two cells 25 and 27 hours post-insemination) are applicable to in vitro fertilisation programmes, thereby increasing the probability of implantation and pregnancy.
Objective: comparing pregnancy rates in patients implanted with embryos originating from early cleavage to those from the pronuclear stage.
Methodology: a retrospective cohort study was carried out. Embryo implantation patients were included who had been attending the FECUNDAR -Biomedicine Centre in Cali, Colombia, from April 2004 to December 2007 who presented a poor responsetothecontrolledovarianstimulationcycle, age factor and/or ovarian failure and pregnancy substitute patients. The sample consisted of 45 patients; 36 patients (79%) were expected to become pregnant in the early-syngamy and cleavage group (group 1) and 9 patients (21%) in the pronuclear group (group 2). Implantation and pregnancy rates were determined and the Chi-square (X2) test was used for comparing both groups.
Results: 183 of the 289 zygotes complied with the proposed parameters (i.e. embryos from early cleavage, syngamy and pronuclear stage 25 and 27 hours post-insemination); 79% of these 183 came from the early cleavage and syngamy stage and 21% from the pronuclear stage. Three embryos on average were transferred to embryo implantation patient; average age was 38, the pregnancy rate obtained when transferring early cleavage embryos was 44% compared to 41% for non-cleaved embryo transfer 25 hours post-insemination. No statistically significant differences were found regarding how pregnancy was induced.
Conclusion: equal success rates were obtained for both procedures in the given study conditions.
Author Biographies
María Esther Estela-Paz
Jaime Saavedra-Saavedra
Profesor Titular, Universidad del Valle. Maestro de la especialidad de Ginecología y Obstetricia del Valle del Cauca. Director general de FECUNDAR, Cali (Colombia).
Martha Norah Moyano
Bióloga. MSc en Epidemiología. Docente de la Universidad del Valle. Epidemióloga FECUNDAR, Cali (Colombia).
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