The relationship between post-capacitated sperm characteristics and human oocyte fertilization in the in-vitro fertilization technique
DOI:
https://doi.org/10.18597/rcog.267Keywords:
spermatic characteristics, fertilización oocitaria, oocyte fertilizationAbstract
Objective: establishing the relationship between post-capacitated sperm characteristics and donated fertilized MII (morphologically mature) oocytes when using the in vitro fertilization (IVF) technique.
Methodology: a descriptive cross-sectional study was carried out between January 2003 and December 2007 at the Centro de Biomedicina Reproductiva del Valle (Fecundar) in Cali (Colombia). Seventysix semen samples taken from patients who had undergone IVF and ovodonation were considered in this study; the percentage of fertilization for these samples was compared, assuming fertilization higher than or equal to 80% to be appropriate. A logistic regression model was used for determining the variables contributing towards presenting a greater probability of oocyte fertilization.
Results: sixty-four of the 76 semen samples fulfilled the inclusion criteria. Statistical analysis revealed that fertilization was affected by postcapacitated sperm motility type a higher than or equal to 25%. The theratozoospermia index (ITZ) and days of sexual abstinence did not affect oocyte fertilization.
Conclusion: post-capacitated sperm motility type a higher than 25% was the variable that most influenced suitable oocyte fertilization in FIV for the individuals tested in this study.
Author Biographies
Yamileth Motato M.
Jaime Saavedra-Saavedra
Martha N. Moyano
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