Inyección intracitoplasmática del espermatozoide (ICSI) una técnica de reproducción asistida con indicaciones

Autores/as

  • Cecilia Hernández

DOI:

https://doi.org/10.18597/rcog.577

Resumen

La inyección intracitoplasmática del espermatozoide (ICSI) ha permitido resolver problemas masculinos severos con excelentes resultados. Sin embargo, la difusión de su técnica llevó a la aplicación en casos donde no existe una indicación clara, desplazando muchas veces a la fertilización in vitro (FIV) sin suficientes argumentos. Esta revisión pretende, partiendo de conceptos fisiológicos de fecundación, llamar la atención sobre los eventos involucrados en el ICSI que transgreden dicha fisiología y constituyen grandes enigmas en cuanto a sus implicaciones en el desarrollo futuro. Las diversas observaciones descritas por los grupos de investigación básica dedicados a estos análisis, si bien no establecen un efecto indeseable inherente a la técnica, dejan abierta la investigación y permiten concluir que el ICSI es una técnica con claras indicaciones que no debe utilizarse indiscriminadamente.

Biografía del autor/a

Cecilia Hernández

Médico Gineco-Obstetra. Universidad Colegio Mayor de Nuestra Señora del Rosario. Bogotá. Colombia.

Referencias bibliográficas

Tesarik J, Sousa M, Testart J. Human oocyte activation after intracytoplasmic sperm injection. Hum Reprod. 1994; 9: 511-518.

Nagy ZP, Liu J, Joris H. Time-course of oocyte activation, pronucleus formation and cleavage in human oocytes fertilized by intracytoplasmic sperm injection. Hum Reprod. 1994; 9: 1743-1748.

Nakano Y, Shirakawa H, Mitsuhachi N. Spatiotemporal dynamics of intracellular calcium in the mouse egg injected with a spermatozoon. Mol Hum Reprod. 1997; 3: 1087-1093.

Ramalho-Santos J, Sotovsky P, Simerly C, Oko R. ICSI choreography: fate of sperm structures after monospermic rhesus ICSI and first cell cycle implications. Human Reprod. 2000; 15 (12): 2610-2620.

Yanagida K, Katayose H, Hirata S. Influence of sperm immobilization on onset of Ca oscillations after ICSI. Hum Reprod. 2001; 16: 148-152.

Williams CJ. Signalling mechanisms of mammalian oocyte activation. Human Reprod Update. 2002; 8 (4): 313-321.

Dale B, Elder K. IVF. 1997. Cambridge University Press: 15-53.

Evans JP. The molecular basis of sperm-oocyte membrane interactions during mammalian fertilization. Hum Reprod Update. 2002; 8 (4): 297-311.

Yanagimachi R. The physiology of reproduction. Mammalian fertilization. In: Knobil, E. and Neill JD. Eds. Raven Press Ltd. New York, USA: 189-317.

Schultz RM. The molecular foundations of the maternal to zygotic transition in the preimplantation embryo. Hum Reprod Update. 2002; 8 (4): 323-331.

Sutovsky P, Oko R, Hewitson L, Schatten G. The removal of the sperm perinuclear theca and its association with bovine oocyte surface during fertilization. Dev Biol. 1997; 188 (1): 75-84.

Schatten G. Cytoskeletal aspects of Assisted fertilization. Seminars in Reproductive Medicine. 2000; 18 (2): 151-159.

Sutovsky P, Hewitson L, Simerly CR. Intracytoplasmic sperm injection for rhesus monkey fertilization results in unusual chromatin, cytoskeletal and membrane events, but eventually leads to pronuclear development and sperm aster assembly. Human Reprod. 1996; 11: 1703-1712.

Tarin JJ, Perez-Albala S, Aguilar A, Minarro J, Cano A. Long-term effects of posovulatory aging of mouse oocytes on offspring: a two-generational study. Biol Reprod. 1999; 61: 1347-1355.

Terada Y, Luetjens CM, Sutovsky P, Schatten G. Atypical decondensation of the sperm nucleus, delayed replication of the male genome, and sex chromosome positioning following intracytoplasmic human sperm injection (ICSI) into golden hamster eggs: Does itself introduce chromosomal anomalies? Fertil Steril. 2000; 74 (3): 454-460.

Bonduelle M, Wilikens A. A follow up study of children born after ICSI with epididymal and testicular spermatozoa and after replacement of cryopreserved embryos obtained after ICSI. Hum Reprod. 1998; 13 (1): 196-207.

Bonduelle M, Wilikens A, Buysse A. Prospective follow-up study of 877 children born after intracytoplasmic sperm injection (ICSI), with ejaculated epididymal and testicular spermatozoa and after replacement of cryopreserved embryos obtained after ICSI. Hum Reprod. 1996; 11 (4): 131-155.

Tarlatzis BC, Bili H. Survey on intracytoplasmic sperm injection: report from the ESHRE ICSI Task Force. Human Reprod. 1998; 13 (Suppl 1): 165-177.

Griffiths TA, Murdoch AP, Herbert M. Embryonic development in vitro is compromised by the ICSI procedure. Hum Reprod. 2000; 15 (7): 1592-1596.

Dumoulin JCM, Coonen E., Bras M. Comparison of in-vitro development of embryos originating from either conventional in-vitro fertilization or intracytoplasmic sperm injection. Hum Reprod. 2000; 15 (2): 402-409.

Fishel S, Aslam I, Lisi F. Should ICSI be the treatment of choice for all cases of in-vitro conception? Hum Reprod. 2000; 15 (6): 1278-1283.

Oehninger S, Gosden RG. Should ICSI be the treatment of choice for all cases of in-vitro conception? Hum Reprod. 2002; 17 (9): 2237-2242.

Ola B, Afnan M, Sharif K. Should ICSI be the treatment of choice for all cases of in-vitro conception? Hum Reprod. 2001; 16 (12): 2485-2490.

Uehara T, Yanagimachi R. Microsurgical injection of spermatozoa into hamster eggs with subsequent transformation of sperm nuclei into male pronuclei. Biol Reprod. 1976; 15: 467-470.

Cómo citar

1.
Hernández C. Inyección intracitoplasmática del espermatozoide (ICSI) una técnica de reproducción asistida con indicaciones. Rev. Colomb. Obstet. Ginecol. [Internet]. 30 de junio de 2003 [citado 25 de mayo de 2022];54(3):157-63. Disponible en: https://revista.fecolsog.org/index.php/rcog/article/view/577

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Publicado

2003-06-30

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